Neurodegenerative Disease Related Proteins Have Negative Effects on SNARE-Mediated Membrane Fusion in Pathological Confirmation
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چکیده
منابع مشابه
Neurodegenerative Disease Related Proteins Have Negative Effects on SNARE-Mediated Membrane Fusion in Pathological Confirmation
Studies showed that synapses are highly-specialized structures for the communication between pre-and postsynaptic neurons (Kaeser and Regehr, 2014). Synaptic vesicles carrying neurotransmitters dock at specialized sites of presynaptic membranes termed active zones, which are closely apposed to postsynaptic densities, and then undergo one or more priming reactions to prepare them to a release-re...
متن کاملImaging single membrane fusion events mediated by SNARE proteins.
Using total internal reflection fluorescence microscopy, we have developed an assay to monitor individual fusion events between proteoliposomes containing vesicle soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and a supported planar bilayer containing cognate target SNAREs. Approach, docking, and fusion of individual vesicles to the target membrane were quantifi...
متن کاملVariable cooperativity in SNARE-mediated membrane fusion.
The soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex drives the majority of intracellular and exocytic membrane fusion events. Whether and how SNAREs cooperate to mediate fusion has been a subject of intense study, with estimates ranging from a single SNARE complex to 15. Here we show that there is no universally conserved number of SNARE complexes involved ...
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Sec1 proteins are critical players in membrane trafficking, yet their precise role remains unknown. We have examined the role of Sec1p in the regulation of post-Golgi secretion in Saccharomyces cerevisiae. Indirect immunofluorescence shows that endogenous Sec1p is found primarily at the bud neck in newly budded cells and in patches broadly distributed within the plasma membrane in unbudded cell...
متن کاملThe Multifaceted Role of SNARE Proteins in Membrane Fusion
Membrane fusion is a key process in all living organisms that contributes to a variety of biological processes including viral infection, cell fertilization, as well as intracellular transport, and neurotransmitter release. In particular, the various membrane-enclosed compartments in eukaryotic cells need to exchange their contents and communicate across membranes. Efficient and controllable fu...
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ژورنال
عنوان ژورنال: Frontiers in Molecular Neuroscience
سال: 2017
ISSN: 1662-5099
DOI: 10.3389/fnmol.2017.00066